|
| Status |
Public on Feb 23, 2012 |
| Title |
CaseC (myeloma) 120 minutes [culture] |
| Sample type |
RNA |
| |
|
| Source name |
CaseC (myeloma) 120 minutes
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: myeloma
sample source: bone marrow culture
protocol: Delayed fixation following spread from the bottom well
time: 120 minutes
|
| Growth protocol |
Cultured bone marrow cells were grown in high cell density imposing cell-cell contact
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen samples were lyzed by adding 300 µl lysis buffer to tubes. Samples stored in RNAlater were fractionated and lyzed by x10 volumes (v/v) lysis buffer. RNA was isolated by MagNA Pure LC RNA Isolation procedure using MagNA Pure instrument (Roche). Integrity of RNA was examined by Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was hybridized onto 1.0 ST Expression Bead Chips
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner.
|
| Description |
culture
CaseC_Culture_120_minutes.CEL
|
| Data processing |
The data were analyzed Affymetrix gene expression console
|
| |
|
| Submission date |
Feb 23, 2012 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Yossi Cohen |
| E-mail(s) |
yosefc@asaf.health.gov.il
|
| Phone |
972-8-9778222
|
| Fax |
972-8-9779772
|
| Organization name |
Asaf Harofeh University Hospital
|
| Department |
Hematology
|
| Street address |
Zeriffin
|
| City |
Be'er Yacob |
| ZIP/Postal code |
97300 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36036 |
Niche modulated versus niche modulating genes in multiple myeloma |
|
