|
Status |
Public on Sep 30, 2012 |
Title |
Cell line 2 |
Sample type |
RNA |
|
|
Source name |
oral squamous cell carcinoma cell lines
|
Organism |
Homo sapiens |
Characteristics |
cell line: HSC2 cell type: oral squamous cell carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
After fresh tissues were homogenized by TissueLyser, total RNA was extracted by ISOGEN.
|
Label |
digoxigenin
|
Label protocol |
Digoxigenin-UTP-labelled cRNA was generated and amplified from 1 mg of total RNA using Applied Biosystems Chemiluminescent RT-IVT Labelling Kit.
|
|
|
Hybridization protocol |
Ten mg of labelled cRNA was hybridized to each pre-hybed microarray at 55℃ for 16 hours.
|
Scan protocol |
After Chemiluminescence detection using Applied Biosystems Chemiluminescence Detection Kit, images were collected by the AB1700 Chemiluminescent Microarray Aanalyzer.
|
Description |
HSC2
|
Data processing |
The robust multichip average (RMA) method was used background correction and normalization.
|
|
|
Submission date |
Feb 27, 2012 |
Last update date |
Sep 30, 2012 |
Contact name |
Koh-ichi Nakashiro |
E-mail(s) |
nakako@m.ehime-u.ac.jp
|
Phone |
+81899605393
|
Organization name |
Ehime University Graduate School of Medicine
|
Department |
Oral and Maxillofacial Surgery
|
Street address |
454 Shitsukawa
|
City |
Toon |
State/province |
Ehime |
ZIP/Postal code |
791-0295 |
Country |
Japan |
|
|
Platform ID |
GPL1426 |
Series (1) |
GSE36090 |
Gene expression profiles in human oral squamous cell carcinoma tissues and cell lines |
|