|
| Status |
Public on Feb 04, 2023 |
| Title |
sp p29 |
| Sample type |
RNA |
| |
|
| Source name |
SP
|
| Organism |
Homo sapiens |
| Characteristics |
stem-like: yes
lmp2a: no
cell line: HK1
|
| Growth protocol |
HK1 were maintained in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS) while C666-1 in fibronectin coated plate in RPMI 1640 medium supplemented with 15% FCS. For sphere-forming assay, C666-1 cells were seeded at 5000 cells/ well in 6-well low attachment plate in serum-free DMEM/F12 (1:1) media supplemented with 5ug/ml Insulin-Transferrin-Selenium-A, 20ng/ml rEGF, 10ng/ml bFGF, 0.4% BSA, 1xB-27 supplement. All cell lines were maintained in 50U/ml penicillin and 50 µg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All RNA samples were extracted by Trizol.
|
| Label |
biotin
|
| Label protocol |
1ug RNA from each sample was labeled according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
All RNA samples were hybridized according to manufacturer's protocol.
|
| Scan protocol |
GeneChip Scanner 3000 7G.
|
| Description |
Gene expression of NPC stem-like cells.
|
| Data processing |
GeneChip Command Console software version 3.0.1.
|
| |
|
| Submission date |
Feb 28, 2012 |
| Last update date |
Feb 04, 2023 |
| Contact name |
Lu Ping Tan |
| E-mail(s) |
luping@imr.gov.my
|
| Organization name |
Institute for Medical Research
|
| Department |
Molecular Pathology Unit
|
| Street address |
Jalan Pahang
|
| City |
Kuala Lumpur |
| ZIP/Postal code |
50588 |
| Country |
Malaysia |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
|
