cultivar: Sangiovese developmental stage: end of véraison protocol: cluster thinned tissue: berries
Treatment protocol
Nine vines per treatment, with the same cluster number at flowering (16 per vine), were selected in a single uniform row and each vine was randomly assigned in three blocks to the two following groups: control (C, no treatment) and cluster thinning (CT, removal of 50% of total clusters per vine at veraison, JD 211).
Growth protocol
Adult Sangiovese (Vitis vinifera L.) vines, clone 12T grafted to SO4, planted in the field in a vineyard in Bologna, Italy (44°30’N, 11°24’E), with North- South oriented rows (vine spacing 1 m × 2.8 m, vertical shoot positioned spur pruned cordon training system.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from the berry tissue without seeds using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO). RNA quality and quantity were determined using a Nanodrop 2000 instrument (Thermo Scientific, Wilmington, DE) and a Bioanalyzer Chip RNA 7500 series II (Agilent, Santa Clara, CA).
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
The microarray was scanned using a ScanArray 4000XL (Perkin-Elmer) at 532 nm (Cy-3 absorption peak) and GenePix Pro7 software (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturers’ instructions. Images were analyzed using NimbleScan v2.5 software (Roche).
Description
Cluster thinned first biological replicate
Data processing
NimbleScan v2.5 software (Roche), produces Pair Files containing the raw signal intensity data for each probe and Calls Files with normalized expression data.
