|
| Status |
Public on Apr 20, 2013 |
| Title |
starved, biological replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
wt mouse starved
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
treatment: starved for 24 h
genetic background: C57/BL6
|
| Treatment protocol |
The liver from two mice (one month old) was starved for 24 hours, while the liver from other two wt mice (one month old) was use as control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs from either starved and control livers were purified by QIAshredder (Qiagen spa, Milano, Italy, catalog no. 79656) and extracted with RNeasy Protect Mini Kit (Qiagen, catalog no. 74126) using standard conditions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the GeneChip IVT labelling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of biotinylated cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse 430A_2 Array
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 .
|
| Description |
Gene expression data from liver of injected mouse
|
| Data processing |
The data were analyzed with the Bioconductor package Affy using RMA method for normalizing and summarizing probe level intensity measurements.
|
| |
|
| Submission date |
Mar 14, 2012 |
| Last update date |
Apr 20, 2013 |
| Contact name |
Rossella De Cegli |
| E-mail(s) |
decegli@tigem.it
|
| Phone |
08119230692
|
| Organization name |
Tigem
|
| Street address |
Via Campi Flegrei 34
|
| City |
Pozzuoli |
| ZIP/Postal code |
80078 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE35015 |
Expression data from injected mice overexpressing TFEB specifically in liver |
| GSE36510 |
Expression data from wild-type mice starved as compared to wild-type control mice |
|
