|
| Status |
Public on Jun 18, 2012 |
| Title |
T-REx293-NPAP1-uninduced-2 |
| Sample type |
RNA |
| |
|
| Source name |
Flp-In™ T-REx™ 293
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293 cells
transgene: NPAP1/C15orf2
treatment: uninduced
|
| Treatment protocol |
Cells were mock treated (H2O)
|
| Growth protocol |
Cells were cultured in DMEM medium supplemented with 10% FCS, 1% penstrep and appropriate concentration of selective antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy mini kit (QIAGEN), treated with DNase I, followed by cleaning-up on spin columns (RNeasy mini kit. Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Amplified cDNA was prepared according to the Ambion WT Expression Kit protocol starting from 200 ng total RNA. Amplified cDNA was fragmented with UDG and APE1 and finally labelled using the Affymetrix WT terminal labelling kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on Human Gene 1.0 ST. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
|
| Scan protocol |
Arrays were scanned in the Affymetrix GeneChip Scanner 3000 wit G7 update
|
| Description |
Gene expression data from cell line T-REx293-NPAP1, not induced, technical replicate 2
|
| Data processing |
The data were analyzed with RMA default setting in Partek GS
|
| |
|
| Submission date |
Mar 16, 2012 |
| Last update date |
Jun 18, 2012 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36575 |
Effect of NPAP1/C15orf2 overexpression on transcriptome of HEK293 cells |
|
