|
| Status |
Public on Sep 30, 2012 |
| Title |
monocyte-derived macrophages, C+LPS_7days_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
monocyte-derived macrophages, Control+LPS, 7 days
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: monocyte-derived macrophages (MDMs)
treatment: control + lipopolysaccharides (LPS)
|
| Treatment protocol |
Cells were treated with 4F (50ug/million cells) for 7 days. Controls were treated with medium alone for 7 days. On day 7, cells were treated with LPS (1ug/mL) for an additional 18 hrs.
|
| Growth protocol |
Primary human monocytes were grown in RPMI medium for 7 days with/without treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the monocyte-derived macrophages using the Ambion mirVANA RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
cRNA was generated by standard methods (Affymetrix) followed by ssDNA fragmentation, end label biotinylation and preparation of hybridization cocktail.
|
| |
|
| Hybridization protocol |
The arrays were hybridized overnight per Affymetrix protocol at 45C, and then washed, stained, and scanned the next day.
|
| Scan protocol |
GeneChips were scanned on an Affymetrix 3000 7G scanner with an autoloader.
|
| Description |
CL-4
|
| Data processing |
The data were analyzed using GeneSpring GX version 11.0.2 software using Affymetrix Exon Expression default settings using RMA16. A baseline transformation to the mean of the Control Samples (GD-C1, GD-C2, GD-C3, and GD-C4) was performed.
|
| |
|
| Submission date |
Mar 29, 2012 |
| Last update date |
Sep 30, 2012 |
| Contact name |
Geeta Datta |
| E-mail(s) |
gdatta@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Street address |
1808 7th Ave S
|
| City |
Birmingham |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36933 |
Regulation of Pattern Recognition Receptors by the Apolipoprotein A-I Mimetic Peptide 4F |
|
