|
| Status |
Public on Apr 25, 2012 |
| Title |
ime4-/- yeast grown in SPO rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ime4-/- cells 4 hrs after transfer to meiosis-inducing media
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: SK1
genotype/variation: ime4-/-
growth medium: SPO
|
| Treatment protocol |
For sporulation yeast cells were transferred to SM (sporulation medium, 1% Kacetate)
|
| Growth protocol |
Yeast are grown vegetatively in YPD (Yeast extract, Peptone, Dextrose) medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 600 ng total RNA (Affymetrix wt_sensetarget_label_manual)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on yeast 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
affymetrix scanner
|
| Data processing |
Feature intensity values from scanned arrays were normalized and reduced to expression summaries using the RMA algorithm. Control probes, probesets with an interquartile range below 0.1 were removed;
|
| |
|
| Submission date |
Mar 30, 2012 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Sharon Moshitch-Moshkovitz |
| E-mail(s) |
sharon.moshkovitz@sheba.health.gov.il
|
| Organization name |
Sheba Medical Center
|
| Department |
Cancer Research Center
|
| Street address |
Laboratory wing
|
| City |
Tel-Hashomer |
| ZIP/Postal code |
52621 |
| Country |
Israel |
| |
|
| Platform ID |
GPL2529 |
| Series (2) |
| GSE36958 |
Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions |
| GSE37005 |
Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq |
|
