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Status
Public on May 04, 2012
Title
PPARg1+Foxp3+Pio#1
Sample type
RNA
Source name
pooled spleen and lymph nodes, T cells, Pparg1, pioglitazone
Organism
Mus musculus Characteristics
genetic background: C57BL/6
Extracted molecule
total RNA
Extraction protocol
TriZol.
Label
Biotin
Label protocol
Affymetrix GeneChip WT Sense Target Labeling and Control Reagents.
Hybridization protocol
Affymetrix GeneChip WT cDNA Synthesis and Amplification Kits.
Scan protocol
Affymetrix GeneChip Scanner.
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
Submission date
Apr 23, 2012
Last update date
May 04, 2012
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246 Series (2)
GSE37533 Expression data of Pioglitazone- or vehicle-treated CD4+FoxP3- T cells transduced with Foxp3+/- Pparg1 (or Pparg2)
GSE37535 PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells
Data table header descriptions ID_REF VALUE RMA-normalized signal intensity
Data table ID_REF VALUE 10344620
15.3351
10344622
211.6427
10344624
427.2333
10344633
546.6624
10344637
177.6833
10344653
17.4265
10344658
328.1101
10344674
10.6273
10344679
39.0198
10344707
576.9613
10344713
448.1999
10344719
62.9265
10344723
60.1119
10344725
52.2469
10344743
24.4558
10344750
315.8552
10344772
22.508
10344789
274.8702
10344797
284.2977
10344799
237.1208
25108 426 Kbytes .Supplementary file Size Download File type/resource GSM921203_EA07068_130423_MOGENE-1_0-ST-V1_NAIVE+PPARG1+FOXP3+PIO_1.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
