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Status
Public on May 04, 2012
Title
PPARg2+Foxp3+Pio#3
Sample type
RNA
Source name
pooled spleen and lymph nodes, T cells, Pparg2, pioglitazone
Organism
Mus musculus Characteristics
genetic background: C57BL/6
Extracted molecule
total RNA
Extraction protocol
TriZol.
Label
Biotin
Label protocol
Affymetrix GeneChip WT Sense Target Labeling and Control Reagents.
Hybridization protocol
Affymetrix GeneChip WT cDNA Synthesis and Amplification Kits.
Scan protocol
Affymetrix GeneChip Scanner.
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
Submission date
Apr 23, 2012
Last update date
May 04, 2012
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246 Series (2)
GSE37533 Expression data of Pioglitazone- or vehicle-treated CD4+FoxP3- T cells transduced with Foxp3+/- Pparg1 (or Pparg2)
GSE37535 PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells
Data table header descriptions ID_REF VALUE RMA-normalized signal intensity
Data table ID_REF VALUE 10344620
16.1746
10344622
222.3443
10344624
422.6465
10344633
647.0953
10344637
209.254
10344653
22.2145
10344658
423.7913
10344674
12.1571
10344679
41.8559
10344707
525.0997
10344713
423.2876
10344719
63.3346
10344723
122.2587
10344725
45.3937
10344743
21.1993
10344750
307.114
10344772
22.1212
10344789
303.473
10344797
244.9989
10344799
290.6398
25108 426 Kbytes .Supplementary file Size Download File type/resource GSM921211_EA07068_130450_MOGENE-1_0-ST-V1_NAIVE+PPARG2+FOXP3+PIO_3.CEL.gz
4.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
