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Status
Public on May 04, 2012
Title
Foxp3+Pio#2
Sample type
RNA
Source name
pooled spleen and lymph nodes, T cells, pioglitazone
Organism
Mus musculus Characteristics
genetic background: C57BL/6
Extracted molecule
total RNA
Extraction protocol
TriZol.
Label
Biotin
Label protocol
Affymetrix GeneChip WT Sense Target Labeling and Control Reagents.
Hybridization protocol
Affymetrix GeneChip WT cDNA Synthesis and Amplification Kits.
Scan protocol
Affymetrix GeneChip Scanner.
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
Submission date
Apr 23, 2012
Last update date
May 04, 2012
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246 Series (2)
GSE37533 Expression data of Pioglitazone- or vehicle-treated CD4+FoxP3- T cells transduced with Foxp3+/- Pparg1 (or Pparg2)
GSE37535 PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells
Data table header descriptions ID_REF VALUE RMA-normalized signal intensity
Data table ID_REF VALUE 10344620
14.6234
10344622
231.4194
10344624
504.0772
10344633
636.6669
10344637
212.2361
10344653
21.7753
10344658
412.7715
10344674
10.4196
10344679
42.4269
10344707
463.7736
10344713
370.5321
10344719
56.0584
10344723
102.2859
10344725
43.3327
10344743
17.4329
10344750
414.852
10344772
22.4095
10344789
331.8358
10344797
264.75
10344799
310.5516
25108 426 Kbytes .Supplementary file Size Download File type/resource GSM921216_EA07068_130427_MOGENE-1_0-ST-V1_NAIVE+FOXP3+PIO_2.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
