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Status
Public on May 04, 2012
Title
Foxp3+PPARg1+GW1929#1
Sample type
RNA
Source name
pooled spleen and lymph nodes, T cells, Pparg1, GW1929
Organism
Mus musculus Characteristics
genetic background: C57BL/6
Extracted molecule
total RNA
Extraction protocol
TriZol.
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit.
Hybridization protocol
Ambion WT Expression Kit.
Scan protocol
Affymetrix GeneChip Scanner.
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
Submission date
Apr 23, 2012
Last update date
May 04, 2012
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246 Series (2)
GSE37534 Expression data of Pioglitazone-, Rosiglitazone-, GW1929- and vehicle-treated CD4+FoxP3- T cells transduced with Foxp3+Pparg1
GSE37535 PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells
Data table header descriptions ID_REF VALUE RMA-normalized signal intensity
Data table ID_REF VALUE 10344620
18.1981
10344622
95.1562
10344624
608.4097
10344633
538.6225
10344637
222.7618
10344653
18.3029
10344658
458.568
10344674
9.5273
10344679
23.1197
10344707
400.9209
10344713
250.1257
10344719
61.5372
10344723
19.7415
10344725
32.5505
10344743
29.3122
10344750
224.1135
10344772
25.0538
10344789
220.2707
10344797
260.0006
10344799
277.7412
25194 426 Kbytes .Supplementary file Size Download File type/resource GSM921221_EA07068_201130_MOGENE-1_0-ST-V1_FOXP3+PPARG1+GW1929_1.CEL.gz
3.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
