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Status
Public on May 04, 2012
Title
Foxp3+PPARg1+ROS1#3
Sample type
RNA
Source name
pooled spleen and lymph nodes, T cells, Pparg1, rosiglitazone
Organism
Mus musculus Characteristics
genetic background: C57BL/6
Extracted molecule
total RNA
Extraction protocol
TriZol.
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit.
Hybridization protocol
Ambion WT Expression Kit.
Scan protocol
Affymetrix GeneChip Scanner.
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
Submission date
Apr 23, 2012
Last update date
May 04, 2012
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246 Series (2)
GSE37534 Expression data of Pioglitazone-, Rosiglitazone-, GW1929- and vehicle-treated CD4+FoxP3- T cells transduced with Foxp3+Pparg1
GSE37535 PPARĪ³ is a major driver of the accumulation and phenotype of adipose-tissue Treg cells
Data table header descriptions ID_REF VALUE RMA-normalized signal intensity
Data table ID_REF VALUE 10344620
16.9346
10344622
123.955
10344624
1261.5891
10344633
608.4894
10344637
282.8446
10344653
15.6953
10344658
598.3991
10344674
10.9226
10344679
24.5322
10344707
606.2923
10344713
283.3049
10344719
65.6958
10344723
24.0501
10344725
41.7502
10344743
21.3488
10344750
378.284
10344772
23.8544
10344789
344.3117
10344797
211.4083
10344799
395.2546
25194 426 Kbytes .Supplementary file Size Download File type/resource GSM921229_EA07068_201135_MOGENE-1_0-ST-V1_FOXP3+PPARG1+ROS1_3.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
