|
| Status |
Public on Sep 14, 2012 |
| Title |
WT_B_1.CEL |
| Sample type |
RNA |
| |
|
| Source name |
CENPK1137D
|
| Organism |
Saccharomyces cerevisiae CEN.PK113-7D |
| Characteristics |
cultivation protocol: Batch
|
| Treatment protocol |
biomass samples were taken at steadystate, cooled down imeediately and centrifuged with cooling, The cell prllets were frozen in liquid nitrogen
|
| Growth protocol |
Batch and Chemostat cultivation on glucose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen cell pellets using FastRNA® Pro RED kit (QBiogene, Inc, USA)
|
| Label |
biotin
|
| Label protocol |
Labelling was done according to the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip yeast2 Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) by A GeneChip Fluidics Station FS-400 and GeneChip Scanner 3000 7G
|
| Description |
SRA accession from the same sample: SRR453566
|
| Data processing |
Probe Logarithmic Intensity Error (PLIER)
|
| |
|
| Submission date |
Apr 26, 2012 |
| Last update date |
Sep 14, 2012 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
inookaew@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE37599 |
Expression data of Saccharomyces cerevisiae CEN.PK.113-7D grew in Batch and Chemostat condition using for comparison of RNA-seq and Microarray data |
|
