|
Status |
Public on Sep 14, 2012 |
Title |
WT_C_8.CEL |
Sample type |
RNA |
|
|
Source name |
CENPK1137D
|
Organism |
Saccharomyces cerevisiae CEN.PK113-7D |
Characteristics |
cultivation protocol: Chemostat
|
Treatment protocol |
biomass samples were taken at steadystate, cooled down imeediately and centrifuged with cooling, The cell prllets were frozen in liquid nitrogen
|
Growth protocol |
Batch and Chemostat cultivation on glucose
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from frozen cell pellets using FastRNA® Pro RED kit (QBiogene, Inc, USA)
|
Label |
biotin
|
Label protocol |
Labelling was done according to the Affymetrix GeneChip Expression Analysis Technical Manual.
|
|
|
Hybridization protocol |
Samples were hybridized with GeneChip yeast2 Arrays (Affymetrix)
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) by A GeneChip Fluidics Station FS-400 and GeneChip Scanner 3000 7G
|
Description |
SRA accession from the same sample: SRR453570
|
Data processing |
Probe Logarithmic Intensity Error (PLIER)
|
|
|
Submission date |
Apr 26, 2012 |
Last update date |
Sep 14, 2012 |
Contact name |
Intawat Nookaew |
E-mail(s) |
inookaew@uams.edu
|
Organization name |
University of Arkansas for Medical Sciences
|
Department |
Department of Biomedical Informatics
|
Street address |
4301 W Markham St
|
City |
Little Rock |
ZIP/Postal code |
72205 |
Country |
USA |
|
|
Platform ID |
GPL2529 |
Series (1) |
GSE37599 |
Expression data of Saccharomyces cerevisiae CEN.PK.113-7D grew in Batch and Chemostat condition using for comparison of RNA-seq and Microarray data |
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