|
| Status |
Public on Nov 25, 2013 |
| Title |
patient1_ovary_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
patient 1, ovarian epithelial cells, cultured, hTERT-immortalized
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 1
origin: ovarian epithelium
cell passage: 2
|
| Growth protocol |
Cells were grown in newly developed WIT-fo medium (described in the associated publication)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNA Mini kits according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were normalized using the vsnrma function in the package vsn.
|
| |
|
| Submission date |
Apr 29, 2012 |
| Last update date |
Nov 25, 2013 |
| Contact name |
Melissa Amy Merritt |
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Biostatistics and Computational Biology
|
| Lab |
Computational Biology and Functional Genomics (Quackenbush)
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215-5450 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE37648 |
Gene signatures of normal hTERT immortalized ovarian epithelium and fallopian tube epithelium (paired cultures from 2 donor patients) |
|
