|
| Status |
Public on Oct 03, 2012 |
| Title |
estradiol + progesterone E2+PROG replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
ZR-75-1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: estradiol + progesterone
cell line: ZR-75-1
|
| Treatment protocol |
Plated 1.5x10e5 cells/well in 6-well dishes for 48h in phenol red-free RPMI 1640 containing 10% hormone stripped FCS and treated for 16h with 10nM E2 or 10nM DHT alone or in combination, or equivalent vehicle (ethanol).
|
| Growth protocol |
ZR-75-1 cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
E2+PROG replicate 3
|
| Data processing |
Data was processed in Illumina Bead Studio using using Rank Invariant normalization. Subsequent data corrections and gene analyses were performed in R/Bioconductor.
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
Oct 03, 2012 |
| Contact name |
Grant Buchanan |
| E-mail(s) |
grant.buchanan@adelaide.edu.au
|
| Organization name |
Basil Hetzel Institute for Translational Health Research, University of Adelaide
|
| Department |
Surgery
|
| Lab |
Cancer Biology Group
|
| Street address |
28 Woodville Road
|
| City |
Woodville South |
| State/province |
South Australia |
| ZIP/Postal code |
5011 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE38132 |
ZR-75-1 microarray expression data |
|
