|
Status |
Public on Oct 03, 2012 |
Title |
medroxyprogesterone acetate MPA replicate 4 |
Sample type |
RNA |
|
|
Source name |
ZR-75-1 cells
|
Organism |
Homo sapiens |
Characteristics |
treatment: medroxyprogesterone acetate cell line: ZR-75-1
|
Treatment protocol |
Plated 1.5x10e5 cells/well in 6-well dishes for 48h in phenol red-free RPMI 1640 containing 10% hormone stripped FCS and treated for 16h with 10nM E2 or 10nM DHT alone or in combination, or equivalent vehicle (ethanol).
|
Growth protocol |
ZR-75-1 cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
MPA replicate 4
|
Data processing |
Data was processed in Illumina Bead Studio using using Rank Invariant normalization. Subsequent data corrections and gene analyses were performed in R/Bioconductor.
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|
|
Submission date |
May 22, 2012 |
Last update date |
Oct 03, 2012 |
Contact name |
Grant Buchanan |
E-mail(s) |
grant.buchanan@adelaide.edu.au
|
Organization name |
Basil Hetzel Institute for Translational Health Research, University of Adelaide
|
Department |
Surgery
|
Lab |
Cancer Biology Group
|
Street address |
28 Woodville Road
|
City |
Woodville South |
State/province |
South Australia |
ZIP/Postal code |
5011 |
Country |
Australia |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE38132 |
ZR-75-1 microarray expression data |
|