|
| Status |
Public on Jun 01, 2013 |
| Title |
LPSTZ pancreas, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
pancreas, low protein diet, STZ injected
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: streptozotocin
|
| Treatment protocol |
Injections were given daily from days 1 to 5 from birth.
|
| Growth protocol |
Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
|
| |
|
| Hybridization protocol |
10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
|
| |
|
| Submission date |
May 24, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Aaron R Cox |
| E-mail(s) |
arcox2@gmail.com
|
| Organization name |
Baylor College of Medicine
|
| Department |
Pediatrics - Endocrinology and Diabetes
|
| Street address |
1102 Bates Ave.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77025 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38215 |
Expression analysis of control and low protein fed mouse offspring subsequently treated with multiple low doses of STZ |
|
