|
Status |
Public on May 20, 2013 |
Title |
Control [G3] |
Sample type |
RNA |
|
|
Source name |
human breast cancer cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 condition: control
|
Treatment protocol |
Where indicated, cells were treated with 10 nM 17-β-estradiol for 6 hours.
|
Growth protocol |
Cells were transfected with siRNAs, grown for 24 h in DMEM containing 10% bovine growth serum then grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 24 hours prior to treatment with estrogen for 6 h.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiazol (Qiagen) according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Cat # IL1791); 200ng total RNA input
|
|
|
Hybridization protocol |
Illumina Whole-Genome Gene Expression Direct Hybridization Assay Guide (11286331)
|
Scan protocol |
Illumina iScan Scanner
|
Data processing |
log2 transformation of signal intensity, quantile normalization, R/Bioconductor, BeadStudio
|
|
|
Submission date |
May 25, 2012 |
Last update date |
May 20, 2013 |
Contact name |
Frank Kramer |
E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
Organization name |
University Medical Center Göttingen
|
Department |
Department of Medical Statistics
|
Street address |
Humboldtallee 32
|
City |
Goettingen |
ZIP/Postal code |
37073 |
Country |
Germany |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE38252 |
Cohesin is required for expression of the estrogen receptor-alpha (ESR1) gene |
|