|
| Status |
Public on May 20, 2013 |
| Title |
siRNA Smc3 + Estrogen [N2] |
| Sample type |
RNA |
| |
|
| Source name |
human breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
condition: siRNA Smc3 + Estrogen
|
| Treatment protocol |
Where indicated, cells were treated with 10 nM 17-β-estradiol for 6 hours.
|
| Growth protocol |
Cells were transfected with siRNAs, grown for 24 h in DMEM containing 10% bovine growth serum then grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 24 hours prior to treatment with estrogen for 6 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiazol (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Cat # IL1791); 200ng total RNA input
|
| |
|
| Hybridization protocol |
Illumina Whole-Genome Gene Expression Direct Hybridization Assay Guide (11286331)
|
| Scan protocol |
Illumina iScan Scanner
|
| Data processing |
log2 transformation of signal intensity, quantile normalization, R/Bioconductor, BeadStudio
|
| |
|
| Submission date |
May 25, 2012 |
| Last update date |
May 20, 2013 |
| Contact name |
Frank Kramer |
| E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
| Organization name |
University Medical Center Göttingen
|
| Department |
Department of Medical Statistics
|
| Street address |
Humboldtallee 32
|
| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE38252 |
Cohesin is required for expression of the estrogen receptor-alpha (ESR1) gene |
|
