|
| Status |
Public on Jun 21, 2012 |
| Title |
DR(0.5% glucose) |
| Sample type |
RNA |
| |
|
| Source name |
Budding yeast cells on DR media (restricted)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4742
genotype: wild-type (Mat alpha; his3Delta1; leu2Delta0; lys2Delta0; ura3Delta0)
diet: restricted
|
| Treatment protocol |
Cells were inoculated into AL or DR liquid media (AL: yeast extract (1%) peptone plus (2%) D-glucose; DR: yeast extract (1%) peptone plus (0.5%) D-glucose).
|
| Growth protocol |
Wild-type yeast cells (BY4742) were inoculated into AL or DR liquid media and incubated overnight on a shaker. These seed cultures were then inoculated into fresh media to an initial OD600 of 0.01. Cells were grown at 30 degree Celsius for 12 hours. The final OD600 of cultures were 0.85 - 0.99.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Three OD600 units of cells were collected and lysed with zymolyase. Total RNA was isolated using the Qiagen RNeasy mini kit according to the manufacturer’s protocol. Total RNA obtained was between 60 to 100 microgram.
|
| Label |
biotin
|
| Label protocol |
see standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
RNA samples were processed and hybridized with GeneChip Yeast genome 2.0 arrays (Affymetrix Inc., Santa Clara, CA) by Expression Analysis (Durham, NC).
|
| Scan protocol |
see standard Affymetrix protocol
|
| Description |
Gene expression data from budding yeast on dietary restriction media.
|
| Data processing |
The hybridization signals were normalized and analyzed with the Affy statistical algorithms by Expression Analysis.
|
| |
|
| Submission date |
Jun 11, 2012 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Daniel Wuttke |
| E-mail(s) |
age@liv.ac.uk
|
| Phone |
4915253306032
|
| Organization name |
University of Liverpool
|
| Department |
Department of Functional and Comparative Genomics
|
| Lab |
Integrative Genomics of Ageing Group
|
| Street address |
Crownstreet
|
| City |
Liverpool |
| State/province |
Meyerside |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE38635 |
Expression data from dietary restricted budding yeast |
|
