|
| Status |
Public on Aug 23, 2012 |
| Title |
Activated T cell blasts (2 days post invitro activation), replicate 5, Rat230_array-B |
| Sample type |
RNA |
| |
|
| Source name |
in vitro T cells day 2
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: CD4+ myelin specific T cells
state: blast
days after antigen challenge: 2
strain: Lewis rat
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Qiagen RNeasy mini kit according to the manufactor instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
CD4+ myelin specific T cells
|
| Data processing |
Gene expression profiles were background corrected and normalized on probe level using the variance stabilization method by Huber et al (Bioinformatics 2002;18:S96–104). Normalized probe intensities were summarized into gene expression levels using the additive model described in Irizarry et al (Nucleic Acids Res 2003;31:15)
|
| |
|
| Submission date |
Jun 11, 2012 |
| Last update date |
Aug 23, 2012 |
| Contact name |
alexander flügel |
| E-mail(s) |
fluegel@med.uni-goettingen.de
|
| Phone |
++49 (0)551 39 13332
|
| Fax |
++49 (0)551 39 13348
|
| Organization name |
Institute for Multiple Sclerosis Research
|
| Department |
Department of Neuroimmunology
|
| Street address |
waldweg 33
|
| City |
Göttingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL342 |
| Series (1) |
| GSE38645 |
Gene expression profiling of CD4+myelin basic protein specific T cells |
|
