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Status |
Public on Aug 23, 2012 |
Title |
Ex vivo sorted T cell from spleen (3 days post adoptive transfer), replicate 1, Rat230_array-B |
Sample type |
RNA |
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Source name |
Spleen day 3
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Organism |
Rattus norvegicus |
Characteristics |
cell type: sorted T cell from spleen strain: Lewis rat age: 6-8 weeks gender: female tissue: spleen
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the Qiagen RNeasy mini kit according to the manufactor instruction
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
CD4+ myelin specific T cells
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Data processing |
Gene expression profiles were background corrected and normalized on probe level using the variance stabilization method by Huber et al (Bioinformatics 2002;18:S96–104). Normalized probe intensities were summarized into gene expression levels using the additive model described in Irizarry et al (Nucleic Acids Res 2003;31:15)
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Submission date |
Jun 11, 2012 |
Last update date |
Aug 23, 2012 |
Contact name |
alexander flügel |
E-mail(s) |
fluegel@med.uni-goettingen.de
|
Phone |
++49 (0)551 39 13332
|
Fax |
++49 (0)551 39 13348
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Organization name |
Institute for Multiple Sclerosis Research
|
Department |
Department of Neuroimmunology
|
Street address |
waldweg 33
|
City |
Göttingen |
ZIP/Postal code |
37073 |
Country |
Germany |
|
|
Platform ID |
GPL342 |
Series (1) |
GSE38645 |
Gene expression profiling of CD4+myelin basic protein specific T cells |
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