|
| Status |
Public on Jan 01, 2015 |
| Title |
Brain_MTX_Methotrexate |
| Sample type |
RNA |
| |
|
| Source name |
Cortical neurons, Methotrexate, NMDA
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cortical neurons from whole brain
neuroprotective molecule: Methotrexate
solvent: water
mechanism: enzyme inhibitor
ratio protection: 1.61
class: protective
|
| Treatment protocol |
Media was replaced with fresh media containing neuroprotective molecules or controls and incubated for one hour. Neurons were then exposed to NDMA in excitotoxic media (hypotonic salt solution) for one hour. Media was replaced with fresh media containing molecule or controls, respectively, and incubated for a further 16hr.
|
| Growth protocol |
Dissociated cortical neurons were cultured on poly-ornithine/poly-lysine coated plates in neurobasal A medium supplemented with B-27 and penicillin/streptomycin. Neurons were cultured for seven days before experimental conditions were introduced.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with clean-up using the QIAGEN RNeasy mini kit in accordance with the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
4060001036_E
MTX
Non-control single sample.
|
| Data processing |
The data were background subtracted and normalized (cubic spline) using the Illumina BeadStudio Gene Expression module v3.1.
|
| |
|
| Submission date |
Jun 13, 2012 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Derek Pappas |
| E-mail(s) |
djpappas75@gmail.com
|
| Organization name |
University of California, San Francisco
|
| Department |
Neurology
|
| Lab |
Jorge Oksenberg
|
| Street address |
675 Nelson Rising Lane, RM 240
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE38701 |
Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules |
|
