|
| Status |
Public on Dec 01, 2012 |
| Title |
Microglia at 12 months biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Microglia at 12 months
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: wild type
age: 12 months
cell type: Flow sorted Microglia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared for the Affymetrix GeneChip Mouse Exon 1.0 ST Arrays using Nugen WT-Ovation One-Direct RNA Amplification System and Encore Biotin Module starting with less than 5 ng of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation and labelling, 5 ug of cDNA were hybridized at 45C for 21 hours. Arrays were washed using Affymetrix fluidics stations and then scanned in an Affymetrix GeneChip Scanner 3000 7G.
|
| Scan protocol |
GeneChips were scanned with an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
1_microglia-1_12m.CEL
|
| Data processing |
The data were analyzed using GeneSpring 11.5. RMA was used for normalization (median polish was used for summarization).
|
| |
|
| Submission date |
Jun 15, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Radu Cojocaru |
| E-mail(s) |
raducojo@gmail.com
|
| Organization name |
Elsevier
|
| Street address |
5635 Fishers Lane, Suite 510
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20852 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE38739 |
Age-related changes in gene expression in retinal microglia |
|
