|
Status |
Public on Dec 01, 2012 |
Title |
Microglia at 24 months biological replicate 1 |
Sample type |
RNA |
|
|
Source name |
Microglia at 24 months
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype/variation: wild type age: 24 months cell type: Flow sorted Microglia
|
Extracted molecule |
total RNA |
Extraction protocol |
extraction protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared for the Affymetrix GeneChip Mouse Exon 1.0 ST Arrays using Nugen WT-Ovation One-Direct RNA Amplification System and Encore Biotin Module starting with less than 5 ng of total RNA.
|
|
|
Hybridization protocol |
Following fragmentation and labelling, 5 ug of cDNA were hybridized at 45C for 21 hours. Arrays were washed using Affymetrix fluidics stations and then scanned in an Affymetrix GeneChip Scanner 3000 7G.
|
Scan protocol |
GeneChips were scanned with an Affymetrix GeneChip Scanner 3000 7G.
|
Description |
7_microglia-11_24m.CEL
|
Data processing |
The data were analyzed using GeneSpring 11.5. RMA was used for normalization (median polish was used for summarization).
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|
|
Submission date |
Jun 15, 2012 |
Last update date |
Dec 01, 2012 |
Contact name |
Radu Cojocaru |
E-mail(s) |
raducojo@gmail.com
|
Organization name |
Elsevier
|
Street address |
5635 Fishers Lane, Suite 510
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20852 |
Country |
USA |
|
|
Platform ID |
GPL6096 |
Series (1) |
GSE38739 |
Age-related changes in gene expression in retinal microglia |
|