|
| Status |
Public on Dec 04, 2013 |
| Title |
Endothelial cell at T0, patient 5 |
| Sample type |
RNA |
| |
|
| Source name |
Human venous endothelial cell before experimental venous hypertension
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Venous Endothelial
treatment: baseline
individual: patient 5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNAqueous Micro Kit (Ambion Life Technologies, Grand Island, NY).
|
| Label |
biotin
|
| Label protocol |
Biotin labeling of 10 micrograms of amplified RNA was performed using the TrueLabeling Kit (SuperArray Bioscience, Frederick, MD) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
According to manufacturer's protocol
|
| Scan protocol |
Arrays were washed, stained and scanned by GeneChip Scanner 300 7G according to the Affymetrix Expression Analysis technical manual. Affymetrix GCOS 1.4 was used as the image acquisition software. The signal, which represents the intensity of each gene, was extracted from the image. The target intensity value from each chip was scaled to 500.
|
| Data processing |
Affymetrix GCOS 1.4 was used as the image acquisition software. Normalization was performed using RMA with default settings.
|
| |
|
| Submission date |
Jun 18, 2012 |
| Last update date |
Dec 04, 2013 |
| Contact name |
Ryan T Demmer |
| E-mail(s) |
rtd2106@columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Epidemiology
|
| Street address |
722 W 168th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE38783 |
Acute venous hypertension induces local release of inflammatory cytokines and endothelial activation in humans |
|
