|
| Status |
Public on Jul 11, 2012 |
| Title |
GBM Xenograft[G76] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
GBM Xenograft[G76]
|
| Organism |
Homo sapiens |
| Characteristics |
age: unknown
gender: unknown
disease state: Glioblastoma
treatment status: unknown
|
| Growth protocol |
Xenograft cell lines were injected intracranially to generate orothotopic tumors. Mice were sacificed when moribound then brains were collected after cold perfusion of PBS. Brains were flash frozen and kept frozen until RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Followed manufactors directions for the GE Healthcare RNA Illustra minspin kit
|
| Label |
Cy5
|
| Label protocol |
Followed protocal as suggested by agilent
|
| |
|
| Channel 2 |
| Source name |
Reference RNA from pooled cellines
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Reference RNA from pooled cellines
|
| Growth protocol |
Xenograft cell lines were injected intracranially to generate orothotopic tumors. Mice were sacificed when moribound then brains were collected after cold perfusion of PBS. Brains were flash frozen and kept frozen until RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Followed manufactors directions for the GE Healthcare RNA Illustra minspin kit
|
| Label |
Cy3
|
| Label protocol |
Followed protocal as suggested by agilent
|
| |
|
| |
| Hybridization protocol |
40 hour hyb as Agilent protocol describes
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.5.1.1).
|
| Description |
Reference
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) No background, linear Lowess
|
| |
|
| Submission date |
Jun 19, 2012 |
| Last update date |
Jul 11, 2012 |
| Contact name |
Brock Armstrong |
| E-mail(s) |
barmstrong@tgen.org
|
| Phone |
602-343-8768
|
| Organization name |
TGen
|
| Department |
Cancer and Cell Biology
|
| Lab |
Brain Tumor Unit
|
| Street address |
445 N. 5th Street
|
| City |
Phoenix |
| State/province |
AZ |
| ZIP/Postal code |
85004 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE38814 |
Glioblastoma Orthotopic Xenograft Transcriptome |
| GSE39242 |
Glioblastoma Orthotopic Xenograft Transcriptome and Glioblastoma Xenograft Comparative Genomic Hybridization Arrays |
|
