|
| Status |
Public on Nov 19, 2014 |
| Title |
Twin without type 2 diabetes, pair 9 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle, no type 2 diabetes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: vastus lateralis muscle
disease state: no type 2 diabetes
twin pair: 9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from human muscle biopsies were extracted with TRI reagent (Sigma-Aldrich, St. Louis, MO).
|
| Label |
Hy3
|
| Label protocol |
1 µg total RNA was labeled with Hy3 using the miRCURY labeling kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The HS-400-Pro microarray hybridization station (Tecan, Grödig, Austria) was used for hybridization to miRCURY LNA miRNA arrays (Exiqon, Vedbaek, Denmark) representing version 9.2 of the Sanger miRBase, according to Exiqon's protocol.
|
| Scan protocol |
Scanning was performed using the ArrayWoRx white-light CCD-based scanner (Applied Precision, Issaquah, WA). ImaGene 8.0 (BioDiscovery, El Segundo, CA) was used for image analysis including flagging of bad-quality spots.
|
| Description |
9n
|
| Data processing |
Data was processed in R using Limma. Background correction was performed using the normexp method with an offset of 0. Between array normalization was performed using quantiles.
|
| |
|
| Submission date |
Jun 20, 2012 |
| Last update date |
Nov 19, 2014 |
| Contact name |
jette bork-jensen |
| Organization name |
Rigshospitalet
|
| Department |
the department of endocrionlogy (diabetes and metabolism)
|
| Street address |
Tagensvej 20
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE38835 |
Differential regulation of microRNAs in skeletal muscle from monozygotic twins discordant for type 2 diabetes |
|
