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Sample GSM971567 Query DataSets for GSM971567
Status Public on Mar 01, 2014
Title Mice fed High fat diet for 8weeks rep1
Sample type RNA
 
Source name Adipose tissue of mice
Organism Mus musculus
Characteristics strain: C57BL/6J
treatment protocol: High fat diet
time: 8weeks
age: 13 weeks
tissue: epididymal adipose tissue
Treatment protocol C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: E8H1
replicate 1
Data processing Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8).
The data were normalised by quantile method using ArrayAssist® (Stratagene, La Jolla, USA) and R statistical language v. 2.4.1.
 
Submission date Jul 21, 2012
Last update date Mar 01, 2014
Contact name Myung-Sook Choi
E-mail(s) mschoi@knu.ac.kr
Phone 821085881950
Organization name Kyungpook National University
Department Food and Nutrition
Lab Nutrigenomics
Street address 1370, Sankyuk-Dong, Buk-gu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platform ID GPL6887
Series (1)
GSE39549 Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_2417611 7.316856
ILMN_2762289 6.722411
ILMN_2896528 12.640241
ILMN_2721178 10.978267
ILMN_2458837 6.535709
ILMN_3033922 12.236519
ILMN_3092673 13.445857
ILMN_1230777 13.11734
ILMN_1246069 12.513364
ILMN_1232042 6.5837045
ILMN_1243193 10.484374
ILMN_2524361 6.5980153
ILMN_1242440 6.80862
ILMN_1233188 8.072931
ILMN_2543688 11.579794
ILMN_1259789 7.6599855
ILMN_2816356 7.226732
ILMN_1224596 7.779823
ILMN_1233643 6.8970923
ILMN_2808939 9.458077

Total number of rows: 45281

Table truncated, full table size 991 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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