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Sample GSM971571 Query DataSets for GSM971571
Status Public on Mar 01, 2014
Title Mice fed High fat diet for 20weeks rep2
Sample type RNA
 
Source name Adipose tissue of mice
Organism Mus musculus
Characteristics strain: C57BL/6J
treatment protocol: High fat diet
time: 20weeks
age: 25 weeks
tissue: epididymal adipose tissue
Treatment protocol C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: E20H2
replicate 2
Data processing Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8).
The data were normalised by quantile method using ArrayAssist® (Stratagene, La Jolla, USA) and R statistical language v. 2.4.1.
 
Submission date Jul 21, 2012
Last update date Mar 01, 2014
Contact name Myung-Sook Choi
E-mail(s) mschoi@knu.ac.kr
Phone 821085881950
Organization name Kyungpook National University
Department Food and Nutrition
Lab Nutrigenomics
Street address 1370, Sankyuk-Dong, Buk-gu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platform ID GPL6887
Series (1)
GSE39549 Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_2417611 6.475691
ILMN_2762289 6.785768
ILMN_2896528 12.339683
ILMN_2721178 10.805292
ILMN_2458837 6.5527563
ILMN_3033922 12.270987
ILMN_3092673 13.28208
ILMN_1230777 12.787894
ILMN_1246069 12.708051
ILMN_1232042 6.6378894
ILMN_1243193 10.643605
ILMN_2524361 6.612935
ILMN_1242440 6.617611
ILMN_1233188 7.9323144
ILMN_2543688 11.461203
ILMN_1259789 7.569161
ILMN_2816356 7.3537626
ILMN_1224596 7.723501
ILMN_1233643 7.0545654
ILMN_2808939 9.125172

Total number of rows: 45281

Table truncated, full table size 992 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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