|
| Status |
Public on Mar 01, 2014 |
| Title |
Mice fed High fat diet for 20weeks rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Adipose tissue of mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
treatment protocol: High fat diet
time: 20weeks
age: 25 weeks
tissue: epididymal adipose tissue
|
| Treatment protocol |
C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: E20H2
replicate 2
|
| Data processing |
Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8).
The data were normalised by quantile method using ArrayAssist® (Stratagene, La Jolla, USA) and R statistical language v. 2.4.1.
|
| |
|
| Submission date |
Jul 21, 2012 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Myung-Sook Choi |
| E-mail(s) |
mschoi@knu.ac.kr
|
| Phone |
821085881950
|
| Organization name |
Kyungpook National University
|
| Department |
Food and Nutrition
|
| Lab |
Nutrigenomics
|
| Street address |
1370, Sankyuk-Dong, Buk-gu
|
| City |
Daegu |
| ZIP/Postal code |
702-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE39549 |
Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity |
|
