|
Status |
Public on Jul 26, 2012 |
Title |
fALS mRNA subject-1 |
Sample type |
RNA |
|
|
Source name |
CD14+CD16- monocytes
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD14+CD16- monocytes age: 61 years Sex: Male disease state: familial amyotrophic lateral sclerosis fALS SOD1 A4V ethnicity: Non-hispanic onset site: limb fvc: 62 alsfrs: 29
|
Extracted molecule |
total RNA |
Extraction protocol |
Blood monocytes were sorted and total RNA was extracted using mirVana RNA isolation kit (Ambion) according to the manufacturer’s protocol
|
Label |
NA
|
Label protocol |
100 ng of total RNA was used in 5 µl with specific mRNA tag probes from Nanostring using the Nanostring GX Human Immunology and Nanostring Human Inflammation assays
|
|
|
Hybridization protocol |
Hybrdization reactions were performed according to the manufacturer's instructions (NanoString.com) with 5 µl of the sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h
|
Scan protocol |
Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed
|
Description |
Nanostring GX Human Immunology and Nanostring Human Inflammation assays detected RNAs in sorted CD14+/CD16- blood-derived monocytes (RNA transcriptom per 100 ng total RNA)
|
Data processing |
The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization
|
|
|
Submission date |
Jul 25, 2012 |
Last update date |
Jul 26, 2012 |
Contact name |
Oleg Butovsky |
E-mail(s) |
obutovsky@rics.bwh.harvard.edu
|
Phone |
1-617-525-5313
|
Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
Department |
Center of Neurologic Diseases
|
Lab |
Dr. Oleg Butovsky
|
Street address |
77 Avenue Louis Pasteur, Office 614
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL15846 |
Series (2) |
GSE39642 |
NanoString nCounter immune-related gene expression in blood sorted CD14+CD16- monocytes from sALS, fALS and HC subjects |
GSE39644 |
Amyotrophic lateral sclerosis |
|