|
| Status |
Public on Jul 26, 2012 |
| Title |
fALS mRNA subject-1 |
| Sample type |
RNA |
| |
|
| Source name |
CD14+CD16- monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD14+CD16- monocytes
age: 61 years
Sex: Male
disease state: familial amyotrophic lateral sclerosis fALS SOD1 A4V
ethnicity: Non-hispanic
onset site: limb
fvc: 62
alsfrs: 29
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood monocytes were sorted and total RNA was extracted using mirVana RNA isolation kit (Ambion) according to the manufacturer’s protocol
|
| Label |
NA
|
| Label protocol |
100 ng of total RNA was used in 5 µl with specific mRNA tag probes from Nanostring using the Nanostring GX Human Immunology and Nanostring Human Inflammation assays
|
| |
|
| Hybridization protocol |
Hybrdization reactions were performed according to the manufacturer's instructions (NanoString.com) with 5 µl of the sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h
|
| Scan protocol |
Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed
|
| Description |
Nanostring GX Human Immunology and Nanostring Human Inflammation assays detected RNAs in sorted CD14+/CD16- blood-derived monocytes (RNA transcriptom per 100 ng total RNA)
|
| Data processing |
The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Jul 26, 2012 |
| Contact name |
Oleg Butovsky |
| E-mail(s) |
obutovsky@rics.bwh.harvard.edu
|
| Phone |
1-617-525-5313
|
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Center of Neurologic Diseases
|
| Lab |
Dr. Oleg Butovsky
|
| Street address |
77 Avenue Louis Pasteur, Office 614
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL15846 |
| Series (2) |
| GSE39642 |
NanoString nCounter immune-related gene expression in blood sorted CD14+CD16- monocytes from sALS, fALS and HC subjects |
| GSE39644 |
Amyotrophic lateral sclerosis |
|
