|
| Status |
Public on Oct 10, 2012 |
| Title |
AsPC-1_vehicle control 24 h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
AsPC-1_vehicle control 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AsPC-1
cell type: human pancreatic ductal adenocarcinoma (PDAC)
|
| Treatment protocol |
AsPC-1 and COLO-357 cells were treated with vehicle control or 5 µM PD-0332991 for 24 h or 72 h
|
| Growth protocol |
AsPC-1 cells were grown in RPMI 1640, COLO-357 cells were grown in DMEM, supplemented with 5% FBS, 100 units/ml penicillin, and 100 µg/ml streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy purification kit
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep Kit
|
| |
|
| Hybridization protocol |
biotin-labeled cRNA samples were hybridized to Illumina HumanHT-12 BeadChips (Version 4.00), using protocol provided by the manufacturer
|
| Scan protocol |
BeadChips were imaged using the Illumina BeadArray Reader
|
| Description |
A_veh_24h_1
|
| Data processing |
Array data analysis was performed using BRB-Array Tools (Version 3.8.1) and the Database for Annotation, Visualization and Integrated Discovery (DAVID, version 6.7)
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Oct 10, 2012 |
| Contact name |
Fang Liu |
| E-mail(s) |
fang.liu@dartmouth.edu
|
| Organization name |
Indiana University
|
| Department |
Endocrinology
|
| Lab |
Korc Lab
|
| Street address |
Walnut street
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE39646 |
Effects of PD-0332991 on gene expression of human pancreatic ductal adenocarcinoma (PDAC) cell lines |
|
