|
| Status |
Public on Aug 03, 2012 |
| Title |
TP53 miR-34a [00KM1474] |
| Sample type |
RNA |
| |
|
| Source name |
mononuclear cells from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
disease: AML
karyotype: complex karyotype
tp53 mutation status: p53 biallelic aberration
mir-34a expression: high
|
| Treatment protocol |
Samples are from untreated patients (de novo AML)
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was purified with a TRIzol-based protocol.
|
| Label |
Biotin
|
| Label protocol |
For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
|
| Scan protocol |
Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
|
| Description |
00KM1474
|
| Data processing |
The data were analyzed with BRB ArrayTools using RMA. Intensity values were log2 transformed
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Aug 04, 2012 |
| Contact name |
Lars Bullinger |
| E-mail(s) |
lars.bullinger@charite.de
|
| Phone |
+49-30-450-553111
|
| Organization name |
Charité
|
| Department |
Hematology, Oncology and Tumorimmunology
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE39730 |
Altered miRNA and gene expression in acute myeloid leukemia with complex karyotype identify networks of prognostic relevance |
|
