|
Status |
Public on Aug 03, 2012 |
Title |
TP53 miR-34a [06PB5792] |
Sample type |
RNA |
|
|
Source name |
mononuclear cells from bone marrow
|
Organism |
Homo sapiens |
Characteristics |
disease: AML karyotype: complex karyotype tp53 mutation status: p53 biallelic aberration mir-34a expression: high
|
Treatment protocol |
Samples are from untreated patients (de novo AML)
|
Growth protocol |
not applicable
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was purified with a TRIzol-based protocol.
|
Label |
Biotin
|
Label protocol |
For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
|
|
|
Hybridization protocol |
Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
|
Scan protocol |
Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
|
Description |
06PB5792
|
Data processing |
The data were analyzed with BRB ArrayTools using RMA. Intensity values were log2 transformed
|
|
|
Submission date |
Jul 30, 2012 |
Last update date |
Aug 04, 2012 |
Contact name |
Lars Bullinger |
E-mail(s) |
lars.bullinger@charite.de
|
Phone |
+49-30-450-553111
|
Organization name |
Charité
|
Department |
Hematology, Oncology and Tumorimmunology
|
Street address |
Augustenburger Platz 1
|
City |
Berlin |
ZIP/Postal code |
13353 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE39730 |
Altered miRNA and gene expression in acute myeloid leukemia with complex karyotype identify networks of prognostic relevance |
|