|
| Status |
Public on Dec 10, 2013 |
| Title |
Liver from STZ-treated mice, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
liver, STZ-treated mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N
genotype: wildtype
tissue: liver
age: 5-6 months
gender: male
treatment: streptozotocin
|
| Treatment protocol |
To induce type 1 diabetes, 65 mg/kg of streptozotocin (STZ) was injected i.p. into 10- to 16-week-old mice on 5 consecutive days while respective controls were mock-injected with citrate buffer. Animals were sacrificed 9-11 weeks after this treatment.
|
| Growth protocol |
Animals were housed under spf conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen) including DNase treatment.
|
| Label |
biotin
|
| Label protocol |
WT Expression Kit (Ambion) and the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit).
|
| Scan protocol |
According to the Affymetrix expression protocol.
|
| Description |
STZ_30
Gene expression data from liver of STZ-treated mice.
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.2) including median polish and Sketch-Quantile normalisation.
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Dec 10, 2013 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE39752 |
Liver adapts mitochondrial function to insulin-resistant and diabetic states in mice |
|
