|
| Status |
Public on Oct 30, 2013 |
| Title |
apoE-Blood-78-weeks-2 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 78 weeks
tissue: blood
|
| Treatment protocol |
Mouse blood was drawn by heart puncture into EDTA buffer, erythrocytes were lyzed, total blood cells were centrifuged, and taken up in Trizol.
|
| Growth protocol |
Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
|
| Label |
Biotin/SAPE with Anti Streptavidin signal amplification
|
| Label protocol |
ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
|
| |
|
| Hybridization protocol |
60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
|
| Scan protocol |
Affymetrix Scanner 3000, single scan
|
| Description |
Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
|
| Data processing |
GCOS 1.4, scaled to 500
|
| |
|
| Submission date |
Aug 06, 2012 |
| Last update date |
Oct 31, 2013 |
| Contact name |
Andreas Johann Richard Habenicht |
| E-mail(s) |
Andreas.habenicht@med.uni-muenchen.de
|
| Organization name |
Ludwig Maximilians Universität München
|
| Department |
Medicine
|
| Lab |
Institute for Cardiovascular Disease Prevention
|
| Street address |
Pettenkoferstraße 9a
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE40156 |
Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice. |
|
