|
Status |
Public on Oct 30, 2013 |
Title |
wt-Blood-78-weeks-1 |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J gender: male age: 78 weeks tissue: blood
|
Treatment protocol |
Mouse blood was drawn by heart puncture into EDTA buffer, erythrocytes were lyzed, total blood cells were centrifuged, and taken up in Trizol.
|
Growth protocol |
Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
|
Label |
Biotin/SAPE with Anti Streptavidin signal amplification
|
Label protocol |
ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
|
|
|
Hybridization protocol |
60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
|
Scan protocol |
Affymetrix Scanner 3000, single scan
|
Description |
Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
|
Data processing |
GCOS 1.4, scaled to 500
|
|
|
Submission date |
Aug 06, 2012 |
Last update date |
Oct 31, 2013 |
Contact name |
Andreas Johann Richard Habenicht |
E-mail(s) |
Andreas.habenicht@med.uni-muenchen.de
|
Organization name |
Ludwig Maximilians Universität München
|
Department |
Medicine
|
Lab |
Institute for Cardiovascular Disease Prevention
|
Street address |
Pettenkoferstraße 9a
|
City |
Munich |
State/province |
Bavaria |
ZIP/Postal code |
80336 |
Country |
Germany |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE40156 |
Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice. |
|