|
| Status |
Public on Sep 11, 2014 |
| Title |
Tumor epthelial component - sample 629 |
| Sample type |
RNA |
| |
|
| Source name |
Microdissected ovarian tumor epthelial component
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Microdissected ovarian tumor epthelial component
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cancer specimens were obtained from the primary tumors of previously untreated ovarian cancer patients at the Brigham and Women’s Hospital (Boston, MA). Normal human ovarian surface epithelium (HOSE) specimens were obtained from the normal ovaries of patients at the time of surgery for benign indications. All specimens were collected under protocols approved by the institutional review boards of the institution.
Frozen sections were affixed onto FRAME slides (Leica, Wetzlar, Germany), fixed in 70% alcohol and stained by 1% methyl green. Air-dried tissue sections were then taken for microdissection using a MD LMD laser microdissecting microscope (Leica). Different types of tissues were dissected and harvested. Total RNA was isolated using the RNeasy Micro kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
RNA samples were amplified and labeled using the two-cycle amplification protocol (Affymetrix).
|
| |
|
| Hybridization protocol |
For each sample, 15 microgram of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array.
|
| Scan protocol |
GeneChip Scanner 3000 (Affymetrix) was used for array scanning and data collection.
|
| Description |
Gene expression data from microdissected ovarian tumor epthelial component
|
| Data processing |
Microarray data analysis software, GeneSpring V.11.0.2 (Agilent Technologies Inc.) was used to perform the primary data analysis. RMA summerization algorithm was used and baseline transformation to median of all samples were performed.
|
| |
|
| Submission date |
Sep 04, 2012 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Tsz-Lun Yeung |
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Department |
Gynecologic Oncology and Reproductive Medicine
|
| Street address |
1515 Holcombe Blvd. Unit 1362
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE40595 |
A cancer associated fibroblasts (CAFs) specific gene signature in high grade serous ovarian cancer |
|
