Sequencing of fosmid clones by 454 GS FLX - SRA

DRX000009: Sequencing of fosmid clones by 454 GS FLX
3 LS454 (454 GS FLX) runs: 1M spots, 259.6M bases, 608.2Mb downloads

Design: 158 fosmid clones were selected and sequenced at once by 454 sequencer.

Submitted by: Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Study: Subsurface mine microbial mat metagenome

This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine.

Sample: DRS000009

Library:

Name: HGMM Fosmid library

Instrument: 454 GS FLX

Strategy: CLONE

Source: GENOMIC

Selection: other

Layout: SINGLE

Construction protocol: The fosmid library was constructed by using CopyControl Fosmid Library Production Kit (AR BROWN co., ltd).

Spot descriptor:

1 forward

Program
454 Basecaller

Runs: 3 runs, 1M spots, 259.6M bases, 608.2Mb

Run	# of Spots	# of Bases	Size	Published
DRR000019	11,052	2.8M	6.6Mb	2011-03-30
DRR000020	486,919	124.4M	293.7Mb	2011-03-30
DRR000021	518,041	132.4M	307.8Mb	2011-03-30

SRA