Limulus polyphemus Lp-13 (offspring) - SRA

SRX259751: Limulus polyphemus Lp-13 (offspring)
1 ILLUMINA (Illumina HiSeq 2000) run: 27.7M spots, 5.2G bases, 3.2Gb downloads

Design: libraries were constructed using genomic DNA extracted from whole limulus offspring. briefly, larvae were lysed and DNA extracted using disposable plastic pestles and eppendorf tubes followed by proteinase K lysis and DNA extraction as per Qiagen DNAeasy kit protocols. Genomic DNA was further cleaned using Zymo DNA clean columns. Illumina libraries were created using Nextera library prep kit as per kit protocols. Illumina libraries from offspring LP-11 through Lp-34 (all created using Nextera kit) were pooled in equimolar amounts (Lp-11-Lp-22, and Lp23-Lp-34 in two separate pools) for sequencing. Each pool in a separate flowcell lane.

Submitted by: RICE UNIVERSITY

Study: Limulus polyphemus Joint Assembly and Mapping

PRJNA187356 • SRP019763 • All experiments • All runs

show Abstracthide Abstract

Construction of a high density genetic map of the Atlantic horseshoe crab.

Sample: offspring 13

SAMN01901862 • SRS407289 • All experiments • All runs

Organism: Limulus polyphemus

Library:

Name: Lp-13 (offspring)

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward102 reverse

Runs: 1 run, 27.7M spots, 5.2G bases, 3.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR809378	27,711,793	5.2G	3.2Gb	2013-04-11

ID:: 363133

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