Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Reaction RNA products were purified by phenol-chloroform extraction and precipitated overnight at -20°C in ethanol. Reaction RNA products were treated with DNase I and size-selected by gel. Ion Total RNA-Seq Kit v2 for strand-specific RNA sequencing. No shearing step was performed in order to preserve the original length of the molecules.