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SRX042501: GBMF Marine Phage Sequencing Project
2 LS454 (454 GS FLX Titanium) runs: 227,148 spots, 110.5M bases, 245Mb downloads

UUID: a8b27f8d-074a-4f7c-af16-246a26d68b52
Design: International collaboration to sequence, assemble, and annotate approximately 200 diverse marine phage/virus genomes and to sequence approximately 50 viral metagenomes from an array of marine environments. DNA sequencing using 454 technology.
Submitted by: Broad Institute (BI)
Study: Pacific Ocean uncultured phage metagenome
show Abstracthide Abstract
ANME Metagenome - Suboxic seawater overlying carbonate mound formed by hydrate crystalization and expansion. Sediments actively releasing methane to the water column; sample description: TFF concentrated; 0.22 micron filtered; CsCl-gradient; DNAse treatment. Amplified via MDA with phi29 polymerase.
Sample: Generic sample from marine metagenome
SAMN00215775 • SRS172706 • All experiments • All runs
Library:
Name: 454-4152
Instrument: 454 GS FLX Titanium
Strategy: WGS
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Spot descriptor:
          barcode
Group tagBasecalls
ViennaTCACAC
11  forward

Experiment attributes: (show all 6 attributes...) (hide...)
analysis_type: DNA Viral 454 Alignment and Assembly
gssr_id: 46567.1
lsid: BROAD:SEQUENCING_SAMPLE:46567.1
material_type: Genomic DNA
project: G3254
work_request: 21185
Pipeline: show...hide...
NameStepProgramVersion
base caller2010-04-17 19:17:48.0454BaseCaller2.3
Runs: 2 runs, 227,148 spots, 110.5M bases, 245Mb
Run# of Spots# of BasesSizePublished
SRR100454223,500109.2M241.8Mb2011-06-30
SRR1072163,6481.2M3.2Mb2011-06-30

ID:
50773

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