GEO DataSets

Expression profiling of vastus lateralis muscles 3 and 48 hours after a 75-minute bout of high-intensity cycling. Results identify transcriptional pathways activated in skeletal muscles recovering from endurance exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 time sets

Platform:

GPL3457

Series:

GSE4247

8 Samples

Download data: GPR

(Submitter supplied) We examined global mRNA expression using cDNA microarrays in skeletal muscle of humans before, and 3h and 48h after a single bout of exhaustive endurance exercise (cycling). Keywords: Time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1679

Platform:

GPL3457

8 Samples

Download data: GPR

(Submitter supplied) 8,432 randomly chosen cDNAs from a 40k Research Genetics human cDNA library were amplified under standard conditions (GeneAmp PCR System 9700) with the help of a volumetric robot (Tecan/Genesis, RSP150 and Tomtec/Quadra 384). Amplified products were transferred to a 384-well plates, purified transferred to 384-well print plates (Genetix), dried and resuspended in print buffer (3xSSC, 1.5M Betaine). more...

Organism:

Homo sapiens

2 DataSets

3 Series

15 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL9454 GPL570

65 Samples

Download data: CEL

(Submitter supplied) We used gene microarray analysis to compare the global expression profile of genes involved in adaptation to exercise training in skeletal muscle from chronically strength/resistance trained (ST), endurance trained (ET) and untrained control subjects (Con). Resting skeletal muscle samples (~100mg) were obtained from the vastus lateralis of 20 subjects (Con n=7, ET n=7, ST n=6; trained groups >8 years specific training). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6042

21 Samples

Download data: GPR

(Submitter supplied) Protein-leucine supplement ingestion following strenuous endurance exercise accentuates skeletal-muscle protein synthesis and adaptive molecular responses, but the underlying transcriptome is uncharacterized. In a randomized single-blind triple-crossover design, 12 trained men completed 100 min of high-intensity cycling then ingested either 70/15/180/30g protein/leucine/carbohydrate/fat (15LEU), 23/5/180/30g (5LEU) or 0/0/274/30g (CON) beverages during the first 90 min of a 240-min recovery period. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

72 Samples

Download data: TXT

(Submitter supplied) Unconditioned thoroughbred geldings were exercised to maximal heart rate or fatigue on an equine high-speed treadmill. Skeletal muscle biopsies were taken from the middle gluteal muscle before, immediately after and four hours after exercise. 

Organism:

Equus caballus

Type:

Expression profiling by array

Platform:

GPL8582

32 Samples

Download data: GPR

(Submitter supplied) Impact of protein ingestion following 1 h intense cycling on the induced transcriptome and signaling in biopsy samples from endurance-trained men, relative to isocaloric control

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6104

39 Samples

Download data: TXT

(Submitter supplied) Background: Muscle responses to exercise are complex, and potentially include acute responses to exercise-induced injury as well as longer-term adaptive training responses. Using Alaskan sled dogs as an experimental model, changes in muscle gene expression were analyzed to better understand the temporal changes that occur after severe exercise. Methods: Dogs were randomly assigned to undertake in a 160 km run (n=9), or to remain at rest (n=4). more...

Organism:

Canis lupus familiaris

Type:

Expression profiling by array

Platform:

GPL6800

13 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to elucidate the transcriptional signatures that distinguish the endurance-trained and the untrained muscle in healthy adult young males, both at baseline as well as in response to an acute bout of exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

48 Samples

Download data: CEL

(Submitter supplied) Age-related declines in cardiorespiratory fitness and physical function are mitigated by regular endurance exercise in older adults. This may be due, in part, to changes in the transcriptional program of skeletal muscle following repeated bouts of exercise. However, the impact of chronic exercise training on the transcriptional response to an acute bout of endurance exercise has not been clearly determined. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

57 Samples

Download data: TXT

(Submitter supplied) Our results suggest that 48h of immobilization increases expression of mRNA for components of the UPP and MT function while decreasing mRNA and protein for components involved in ECM integrity. We hypothesized that 48h of immobilization would increase gene expression and respective protein products for components of the ubiquitin proteasome pathway (UPP). Keywords: stimulus or stress design

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2083

Platform:

GPL570

10 Samples

Download data: CEL

Analysis of vastus lateralis muscle after knee immobilization for 48 hours. Limbs immobilized due to injury or illness experience significant muscle atrophy within 1 or 2 weeks. Results provide insight into the mechanisms regulating the rapid protein degradation in immobilized skeletal muscles.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 individual, 2 stress sets

Platform:

GPL570

Series:

GSE5110

10 Samples

Download data: CEL

(Submitter supplied) We sought to determine the skeletal muscle genome-wide DNA methylation and mRNA respons-es to one bout of lower-load (LL) versus higher-load (HL) resistance exercise. Trained col-lege-aged males (n=11, 23±4 years old, 4±3 years self-reported training) performed LL or HL bouts to failure separated by one week. The HL bout (i.e., 80 Fail) consisted of four sets of back squats and four sets of leg extensions to failure using 80% of participants estimated one-repetition maximum (i.e., est. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL21145

64 Samples

Download data: CSV, IDAT, PDF

(Submitter supplied) Experiment designed to identify differences in gene expression patterns in previously sedentary individuals before an endurance exercise training program. This series compares 2 groups of individuals that display high and low insulin sensitivity (SI) response (HSIR and LSIR) after 20 weeks of exercise (a 4-fold difference in SI response). There were no differences before training for SI, sex, age, BMI or body fat in the individuals integrating the LSIR and HSIR groups. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2234

Platform:

GPL1412

6 Samples

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Comparison of vastus lateralis muscles of previously sedentary individuals with improved insulin sensitivity (SI) after 20 weeks of exercise to those with no change in SI. Results provide insight into the mechanisms involved in the improvement in SI induced by regular exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 protocol sets

Platform:

GPL1412

Series:

GSE1718

6 Samples

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(Submitter supplied) We investigated the short and long term effects of electrically induced exercise on mRNA expression of human paralyzed muscle. We developed an exercise dose that activated the muscle for 0.6% of the day. The short term effects were assessed 3 hours after a single dose of exercise, while the long term effects were assessed after training 5 days per week for at least one year (adherence 81%). A single dose of electrical stimulation increased the mRNA expression of transcriptional, translational, and enzyme regulators of metabolism important to shift muscle toward an oxidative phenotype (PGC-1a, NR4A3, IFRD1, ABRA, PDK4). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

20 Samples

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(Submitter supplied) 10 male subjects performed ~45 min one-legged cycling and 4 x 7 maximal concentric-eccentric knee extensions for each leg 15 min later. Thus, one limb performed aerobic and resistance exercise (AE+RE), while the opposing leg did resistance exercise only (RE). Biopsies were obtained from m. vastus lateralis of each leg 3 h after the resistance exercise bout. Gene expression analysis was carried out on the Affymetrix HuGene-2.1-st platform.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

20 Samples

Download data: CEL

(Submitter supplied) Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3271

6 Samples

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(Submitter supplied) We sequenced mRNA in vastus lateralis muscle samples from two amateur endurance-trained males (V’O2max 57 ml/min/kg) before and after acute endurance exercise

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: CSV