GEO DataSets

(Submitter supplied) Little is known about genes that promote melanoma cell growth and proliferation. siRNAs may be used to address the role of individual genesin these processes. RNAi library screens were used in the past to gain a comprehensive overview of all genes involved in cell growth, proliferation, migration and other cellular processes. A large-scale loss-of-function screen for eight different melanoma cell lines was performed using a pooled lentiviral shRNA library (GeneNet Human 50K lentiviral shRNA Library,cat#SI206B-1, System Biosciences) to identify genes relevant for melanoma cell growth and proliferation. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL17118

16 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) 4T1 mouse mammary carcinoma cells have an autocrine FGFR active loop leading to constitutive activation of downstream signaling pathways. We found that FGFR inhibitors have a strong effect on 4T1 tumors in-vivo. We used microarray to understand the contribution of FGFR signaling to the tumor formation upon TKI258 treatment.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) 4T1 mouse mammary carcinoma cells have an autocrine FGFR active loop leading to constitutive activation of downstream signaling pathways. We found that FGFR inhibitors have a strong effect on the proliferation and survival of these cells. We used microarray to understand the contribution of FGFR signaling to the tumorigenic phenotype of the 4T1 cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) We investigated whether methylation in primary melanomas is associated with tumor pathologic features or defines subgroups of primary melanomas

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL9183

47 Samples

Download data: TXT

(Submitter supplied) We have developed a screening platform for the isolation of genetic entities involved in metastatic reactivation. Retroviral libraries encoding cDNAs from highly metastatic breast cancer cells or pooled microRNAs were transduced into breast cancer cells that become dormant upon infiltrating the lung. Upon inoculation in the tail vein of mice, the cells that had acquired the ability to undergo reactivation generated metastatic lesions. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL7732

12 Samples

Download data: TXT

(Submitter supplied) We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4840

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of lungs from CCR5-deficient mutants 6 to 48 hrs after tail vein injection with B16-F10 melanoma cells. Lungs of CCR5-deficient mutants develop fewer metastatic nodules than in wild type animals. Results provide insight into the molecular mechanisms underlying CCR5-dependent metastasis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 time sets

Platform:

GPL1261

Series:

GSE51422

6 Samples

Download data: CEL

(Submitter supplied) Gene expression microarrays were used to compare A375 cells treated with a control siRNA or an siRNA targeting ERK3 (MAPK6).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

6 Samples

Download data: CEL, CHP

(Submitter supplied) To identify factors preferentially necessary to drive tumor expansion we performed parallel in vitro and in vivo negative selection shRNA screens. Melanoma cells harboring shRNAs targeting several DNA Damage Response (DDR) kinases had a greater selective disadvantage in vivo than in vitro, indicating an essential contribution of these factors during tumor expansion. In growing tumors, DDR kinases were activated following hypoxia. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL11154

14 Samples

Download data: TXT

(Submitter supplied) Purpose: Asess the transcritpional changes induced upon RAB7 knock-down in melanoma (SK-Mel-28 and UACC-62) and in colon cancer (HCT-116) cell lines. Methods: mRNA profiles of tumor cell lines (SK-Mel-28, UACC-62, HCT-116) stably expressing scrambled shRNA or RAB7 shRNA (harvested at day 3 after lentiviral infection) were generated by deep sequencing, using three biological replicates per condition. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10999

18 Samples

Download data: TXT

(Submitter supplied) The functional role of tumor cell-expressed Angpt2 still remains elusive. Here, we used mouse melanoma cells which have endgeneous Angpt2 expression and invesitgated the functional role of tumor cell-derived Angpt2. Total RNA from control and Angpt2 silenced mouse melanoma cells (RET) was isolated and was further utilized for microarray analysis using Affymetrix Mouse Gene 2.0 ST array.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL

(Submitter supplied) Mouse genetic crosses were established between the PyMT model of metastatic breast cancer and the G7 generation of the Diversity Outcross (DO). Tumors were harvested from the animals for gene expression analysis to identify genes association with progression to distant metastatic disease.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

159 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

267 Samples

Download data: CEL, CHP

(Submitter supplied) Mouse genetic crosses were established between the PyMT model of metastatic breast cancer and the G5 generation of the Diversity Outcross (DO). Tumors were harvested from the animals for gene expression analysis to identify genes associated with progression to distant metastatic disease.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

133 Samples

Download data: CEL, CHP

(Submitter supplied) The goal of this study was to compare the gene expression profiles of a highly versus poorly tumorigenic murine melanoma. B16-F1 is a well-characterized murine melanoma cell line that grows progressively in hosts, both as primary tumors in subcutaneous tissue and as metastatic lesions in internal organs. D5.1G4 is a chemically mutated variant of B16 melanoma that grows with significantly slower kinetics than its wild-type counterpart. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

6 Samples

Download data: TXT