GEO DataSets

Analysis of Tuberous sclerosis complex 1 (Tsc1) deficient CD4 T cells after T cell receptor stimulation using anti-CD3-CD28. Tsc1 is an mTOR signaling modulator involved in naive T cell quiescence and immune homeostasis regulation. Results provide insight into the role of Tsc1 in T cell quiescence.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL11180

Series:

GSE29797

12 Samples

Download data: CEL

(Submitter supplied) The mechanisms that regulate T cell quiescence are poorly understood. We report that tuberous sclerosis complex 1 (Tsc1) establishes a quiescent program in naïve T cells by controlling cell size, cell cycle entry, and responses to T cell receptor stimulation. Loss of quiescence predisposed Tsc1-deficient T cells to apoptosis that depleted conventional T cells and invariant natural killer T cells. Loss of Tsc1 function dampened in vivo immune responses to bacterial infection. Tsc1-deficient T cells exhibited increased mTORC1 but diminished mTORC2 activities, with mTORC1 activation essential for the disruption of immune homeostasis. Therefore, Tsc1-dependent control of mTOR is crucial in establishing naïve T cell quiescence to facilitate adaptive immune function.

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4434 GDS4572

Platform:

GPL11180

16 Samples

Download data: CEL

Analysis of naïve CD4 and CD8 T cells deficient for Tuberous sclerosis complex 1 (Tsc1). Tsc1 tumor suppressor is an mTOR signaling modulator involved in naive T cell quiescence and immune homeostasis regulation. Results identify a Tsc1-dependent gene signature in naive T cells.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 cell type, 2 genotype/variation sets

Platform:

GPL11180

Series:

GSE29797

10 Samples

Download data: CEL

(Submitter supplied) Memory CD8+ T cells are an essential component of protective immunity. Signaling via mechanistic target of rapamycin (mTOR) has been implicated in the regulation of the differentiation of effector and memory T cells. However, little is understood about the mechanisms that control mTOR activity, or the effector pathways regulated by mTOR, in this process. We describe here that tuberous sclerosis 1 (Tsc1), a regulator of mTOR signaling, plays a crucial role in promoting the differentiation and function of memory CD8+ T cells in response to Listeria monocytogenes infection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

9 Samples

Download data: CEL

(Submitter supplied) Rationale: Increased matrix metalloproteinase (MMP) activity has been implicated in the pathogenesis of lymphangioleiomyomatosis (LAM). Objectives: To investigate how TSC1 or TSC2 deficiency alters MMP expression and regulation. Methods: We studied immortalized cells that lack TSC2 derived from an angiomyolipoma (AML) of a LAM patient, and a TSC2 add back derivative; and murine embryonic fibroblast cells that lack Tsc1 or Tsc2 and respective controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2895

9 Samples

Download data

(Submitter supplied) Tuberous sclerosis complex (TSC), an autosomal dominant disorder caused by mutations in either TSC1 or TSC2, exhibits white matter abnormalities including CNS myelin deficits. however, underlying mechanisms are not fully understood. Here we find that, unexpectedly, constitutive activation of mTOR signaling caused by Tsc1 deletion in the oligodendrocyte lineage results in severe myelination defects and oligodendrocyte cell death. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: FPKM_TRACKING, TXT

(Submitter supplied) Activation of mTOR signaling by Tsc1 ablation during early developmental stages increases SC proliferation and delays radial sorting, while causing hypomyelination in the PNS. However, knockout Tsc1 in myelinating SCs at postnatal stages promotes myelin overgrowth and outfoldings. Sustained mTOR pathway leads to activation of the PLK signaling pathway. mTOR attenuation or pharmacological inhibition of PLK1 could partially rescue myelination defects in Tsc1 mutant sciatic nerves. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: XLS

(Submitter supplied) LKB1 encodes a Ser/Thr kinase and acts as an evolutionarily conserved sensor of cellular energy status in eukaryotic cells. LKB1 functions as the major upstream kinase to phosphorylate AMPK and 12 other AMPK-related kinases, which is required for their activation in many cellular contexts. Once activated, AMPK and AMPK-related kinases phosphorylate a diverse array of downstream effectors to switch on ATP-generating catabolic processes and switch off ATP-consuming anabolic processes, thus restoring energy balance during periods of energetic stress. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

7 Samples

Download data: CEL

(Submitter supplied) The mammalian FoxO transcription factors - FoxO1, FoxO3, FoxO4 - function in the nucleus to direct transcription of specific gene targets governing cellular survival, proliferation, metabolism, differentiation and oxidative defense. Activation of PI3K by extracellular growth factors leads to AKT-mediated phosphorylation of FoxO1, FoxO3 and FoxO4, resulting in their sequestration in the cytoplasm such that they are unable to regulate their gene targets. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells

Organism:

Mus musculus

Type:

Other

Platform:

GPL21103

10 Samples

Download data: TXT

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in regulatory T cells

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

4 Samples

Download data: TAR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL23038

52 Samples

Download data: CEL, TAR

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21103

8 Samples

Download data: TSV

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

16 Samples

Download data: CEL

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

8 Samples

Download data: CEL

(Submitter supplied) Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

6 Samples

Download data: CEL