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Platform GPL6606 Query DataSets for GPL6606
Status Public on Jun 26, 2008
Title OSU-CCC (Ohio State University microRNA microarray v2),
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer Microarray Shared Resource, Comprehensive Cancer Center, The Ohio State University (OSU-CCC)
Manufacture protocol Manufacured using GE Healthcare's CodeLink (TM) 3-D Gel Matrix Technology. CodeLink(tm) Activated Slides are designed to covalently immoblize amine-modified DNA for microarrays. The slides are coated with a 3-D surface chemistry comprised of a long-chain, hydrophilic polymer containing amine-reactive groups. This polymer is covalently crosslinked to itself and to the surface of the slide. The crosslinked polymer, combined with end-point attachment, orients the immobilized DNA, and holds it away from the surface of the slide. The 1x printing buffer consists of 50 mM sodium phosphate at pH 8.5. Codelink slides are manufactured by SurModics and were formally sold as 3D-Link(tm) Activated Slides. ¹ Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions that is fitted with a printhead and 16 SMP3 print tips from Telechem. The microRNA microarray probe set is based on 40mer oligonucleotides from Sigma-Genosys. ¹ Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) to a normalized 25 micromolar concentration. The miRNA microarray spot densities are 200 microns (center-to-center). The microarrays are printed on Codelink Activated Slide (PN300011)) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing
 
 
Submission date Mar 19, 2008
Last update date Jun 26, 2008
Contact name Marcos Malumbres
E-mail(s) mmm@cnio.es
Organization name Centro Nacional de Investigaciones Oncológicas (CNIO)
Department Molecular Oncology
Lab Cell Division and Cancer
Street address Melchor Fernandez Almagro 3
City Madrid
ZIP/Postal code E-28029
Country Spain
 
Samples (12) GSM276097, GSM276098, GSM276099, GSM276100, GSM276101, GSM276102 
Series (2)
GSE10861 T-cell lymphoblastic lymphomas induced by gamma-irradiation
GSE10891 Gamma-irradiation induced T-cell lymphomas (miRNA study)

Data table header descriptions
ID
NAME MicroRNA name
miRNA_ID miR name assigned in miRBase
mIRBase ID miRBase code
SEQUENCE Oligonucleotide sequence printed in the array
SPOT_ID

Data table
ID NAME miRNA_ID mIRBase ID SEQUENCE SPOT_ID
Mcd100 left Mcd100 left CTTGGAGGCTGTGGCAATGGCATCTCCAAACTCCGGCAGA
Mcd107 right Mcd107 right AAATGACTAGAATCCGCCCTTCCTGTGCAGTGAGCAAATT
Mcd109 left Mcd109 left CTTCGGTGCTGCTCCAGTGTTTGGCAGCCCTCCTACTTTT
Mcd109 right Mcd109 right CCTGGGTTTGGAGGGGTGCCAGCATTCGGTTCAGCCCCAG
Mcd111 left Mcd111 left CAGGCAGGGGATTTGAGGAGCGGTTGTGTGATTTGTACAG
Mcd111 right Mcd111 right ATTAGGGTAATTGCACGATATTTTCCCAAATTTCTCCTGC
Mcd113 right Mcd113 right AGCCATCAGCTTTTCCCAGCTGCTCATTAATTCCCAGAAA
Mcd115 left Mcd115 left CTGTCTGGCCATTTCGGGTTTTCCAGTCCGATGCTCCTGA
Mcd122 right Mcd122 right GTATGCAATGCACCCGGGCAAGGATTTGGGGAAGGTGAGC
Mcd124 left Mcd124 left TAATTGGTCAGGAAAGACACTTTTTATATTGTCAAGTGGC
Mcd124 right Mcd124 right AAGCCACTAATTCTTGACTGTGAAACTGCTTTTCCTGGGC
Mcd133 right Mcd133 right GGTTGCAGTGAGGTCAATCAGATGCAGTCAAATGGCAGAG
Mcd134 left Mcd134 left CTGAAATCGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTC
Mcd139 right Mcd139 right GTACTTCACTGAAAATTACCAGGCTTCTCTGTGGAGGATG
Mcd14 left Mcd14 left CTCCTCTCAGGGAAGCTTTTTGCTCGCGTTATGTTTCTCA
Mcd14 right Mcd14 right ATCCGAATATAAGACGAACAAAAGGTTTGTCTGAGGGCTG
Mcd140 left Mcd140 left TCTCCCCAAGGACTTATACCACCATTTGATTTAATTTTTA
Mcd140 right Mcd140 right TTAATCAAAAATAGATCAAATGGTGGTGGTCTCTCCGGGG
Mcd141 left Mcd141 left CTCTGTGTGGCTGCTGCTGGCTCAGACCCCAGCCCACCTT
Mcd141 right Mcd141 right GCGGTGGGTTTGGGTTTTGGCTGGCACATTCACCAAGGGG

Total number of rows: 960

Table truncated, full table size 75 Kbytes.




Download family Format
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Supplementary data files not provided

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