|
| Status |
Public on Jun 26, 2008 |
| Title |
Wild-type_thymus_C3 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from wild-type thymus
|
| Organism |
Mus musculus |
| Characteristics |
Wild-type
|
| Treatment protocol |
4-week-old mice of both sexes were exposed to four weekly doses of 1.75 Gy/dose of ionizing gamma
|
| Growth protocol |
Tumors develop in vivo after irradiation of animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by combination of Trizol reagent (Invitrogen) without further purification. The quality of the RNA was evaluated using the BioAnalyzer system (Agilent).
|
| Label |
Cy3
|
| Label protocol |
Five micrograms of the test RNAs was labeled with fluorescent Cy3 (Amhersam, Sunnyvale), respectively using the SuperScript II RNase H Reverse Transcriptase KIT (Invitrogen, USA).
|
| |
|
| Hybridization protocol |
Hybridizations were performed at 55°C for 17 h using the SlideHyb Microarray Hybridization buffer (Ambion).
|
| Scan protocol |
the slides were scanned in an Agilent G2565BA scanner.
|
| Description |
Normal thymus-control
|
| Data processing |
Images were then analyzed with GenePix 5.1 (Axon Instruments, Inc., Union City, CA) and the TM4 software
|
| |
|
| Submission date |
Mar 19, 2008 |
| Last update date |
Jun 26, 2008 |
| Contact name |
Marcos Malumbres |
| E-mail(s) |
mmm@cnio.es
|
| Organization name |
Centro Nacional de Investigaciones Oncológicas (CNIO)
|
| Department |
Molecular Oncology
|
| Lab |
Cell Division and Cancer
|
| Street address |
Melchor Fernandez Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
E-28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6606 |
| Series (2) |
| GSE10861 |
T-cell lymphoblastic lymphomas induced by gamma-irradiation |
| GSE10891 |
Gamma-irradiation induced T-cell lymphomas (miRNA study) |
|
