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| Status |
Public on Dec 31, 2008 |
| Title |
AhR activation by TCDD in the ET, cortical epithelial cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Over activation of the aryl hydrocarbon receptor (AhR) by TCDD results ampng other phenotypes in severe thymic atrophy accompanied by immunosuppression. The link between thymic atrophy, skewed thymocyte differntiation and immunosuppression is still not fully resolved. This study investigates the TCDD elicted exprssion changes in the ET, cortical thymus epithelial cell line.
Keywords: TCDD, AhR, thymic epithelial cells, thymic involution, thymus atrophy
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| Overall design |
ET cells were grown to 80-90% confluence and treated with 5nM TCDD or solvent control (1,4-Dioxane, 0,05%V). 2h,4h and 6h after treatment, RNA was isolated using TRIzol and gene expression measured. All experiments wer done in biological duplicates.
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| Contributor(s) |
Frericks M, Burgoon LD, Zacharewski T, Esser C |
| Citation(s) |
18691609 |
| |
| Submission date |
Jan 14, 2008 |
| Last update date |
May 04, 2018 |
| Contact name |
Markus Frericks |
| E-mail(s) |
fremar5@web.de
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| Phone |
+49(0)211 3389253
|
| Fax |
+49(0)211 3190910
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| Organization name |
Institute for Environmental Research
|
| Department |
Immunology
|
| Street address |
Auf'm Hennekamp 50
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA108369 |
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